Determination of metabolites of phloretin in rats using UHPLC-LTQ-Orbitrap mass spectrometry
نویسندگان
چکیده
Purpose: To study the metabolites of phloretin in vivo using ultra-high performance liquid chromatography linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap).
 Methods: After administration (50 mg/kg; oral route) to six rats, blood samples were taken from each animal. Each sample was then subjected solid-phase extraction prepare it for chromatographic/spectroscopic analysis. Finally, analyzed UHPLC-LTQOrbitrap with a negative-mode electrospray ionization source.
 Results: Based on measurements, chromatographic retention times, and MS2 fragmentation ions, we detected identified 16 drug rats. Metabolic reactions included glucosylation glucuronide conjugation, diglucuronide sulfate hydroxylation.
 Conclusion: The findings provide better understanding metabolism metabolites, new information about their effective forms, pharmacological actions, metabolic fate, toxic actions vivo.
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ژورنال
عنوان ژورنال: Tropical Journal of Pharmaceutical Research
سال: 2021
ISSN: ['1596-5996', '1596-9827']
DOI: https://doi.org/10.4314/tjpr.v18i10.24